Institut Langevin - Ondes et Images : Biophotonics

Our group is interested in the development of far-field optical techniques for the detection of biomolecules and nanoscale pollutants, as well as for the sub-wavelength imaging of cellular environments. In particular, we develop new approaches to super-resolution microscopy, plasmon-based optical biosensors and interferometric microscopy to detect viruses and image biological tissue. Furthermore, we investigate bioinspired self-assembly strategies for high-scale and low-cost nanofabrication techniques in photonics. For more information :
– Optical Antennas
– Super-Resolution Microscopy
– Full-field Optical Coherence Tomography

Recent publications

Nearfield control over magnetic light-matter interactions Reynier, B., E. Charron, O. Markovic, B. Gallas, A. Ferrier, S. Bidault, and M. Mivelle Light: Science and Applications 14, no. 1 (2025) Résumé: Light-matter interactions are frequently perceived as predominantly influenced by the electric field, with the magnetic component of light often overlooked. Nonetheless, the magnetic field plays a pivotal role in various optical processes, including chiral light-matter interactions, photon-avalanching, and forbidden photochemistry, underscoring the significance of manipulating magnetic processes in optical phenomena. Here, we explore the ability to control the magnetic light and matter interactions at the nanoscale. In particular, we demonstrate experimentally, using a plasmonic nanostructure, the transfer of energy from the magnetic nearfield to a nanoparticle, thanks to the subwavelength magnetic confinement allowed by our nano-antenna. This control is made possible by the particular design of our plasmonic nanostructure, which has been optimized to spatially decouple the electric and magnetic components of localized plasmonic fields. Furthermore, by studying the spontaneous emission from the Lanthanide-ions doped nanoparticle, we observe that the measured field distributions are not spatially correlated with the experimentally estimated electric and magnetic local densities of states of this antenna, in contradiction with what would be expected from reciprocity. We demonstrate that this counter-intuitive observation is, in fact, the result of the different optical paths followed by the excitation and emission of the ions, which forbids a direct application of the reciprocity theorem.
Achiral Magnetic Photonic Antenna as a Tunable Nanosource of Chiral Light Cui, L., X. Yang, B. Reynier, C. Schwob, S. Bidault, B. Gallas, and M. Mivelle ACS Photonics 10, no. 11, 3850-3857 (2023) Résumé: Sensitivity to molecular chirality is crucial for many fields, from biology and chemistry to the pharmaceutical industry. By generating superchiral light, nanophotonics has brought innovative solutions to reduce the detection volume and increase sensitivity at the cost of a nonselectivity of light chirality or a strong contribution to the background. Here, we theoretically propose a simple achiral plasmonic resonator based on a rectangular nanoslit in a thin metallic layer behaving as a magnetic dipole to generate a tunable nanosource of purely chiral light working from the UV to the infrared. This nanosource is free of any background, and the sign of its chirality is externally tunable in wavelength and polarization. These unique properties, resulting from the coupling between the incident wave and the magnetic dipolar character of our nanoantenna, coupled with a method of Fluorescent Detected Circular Dichroism (FDCD), shown to be 2 orders of magnitude more sensitive than classical circular dichroism measurements, thus provide a platform with deep subwavelength detection volumes for chiral molecules and a roadmap for optimizing the signal-to-noise ratios in circular dichroism measurements to reach single-molecule sensitivity.

Rapid On-Site Histopathological Analysis of Kidney Biopsy With Dynamic Full-Field Optical Coherence Tomography Zuccarelli, L., Q. Bernard, G. Tarris, L. Martin, M. Funes De La Vega, A. Jacq, J. M. Rebibou, C. Tinel, C. Boccara, O. Thouvenin, J. M. Chassot, M. Rabant, J. Zuber, C. Legendre, J. P. Quenot, M. N. Peraldi, L. Amrouche, A. Scemla, N. Chavarot, D. Anglicheau, M. Legendre, and T. Maldiney Kidney International Reports (2025) Résumé: Introduction: Kidney histology preparation requires a multistep process that is usually responsible for delayed results. This study introduces dynamic full-field optical coherence tomography (D-FF-OCT) as a label-free alternative to overcome the limitations of traditional histopathology for on-site kidney pathology assessment. Methods: Two patient cohorts were considered, with a total of 31 patients included in the study; one cohort involved patients requiring biopsy of transplant kidney, and the other involved patients requiring biopsy of native kidney. The clinical and biological data were prospectively collected. Histopathological analysis of kidney biopsies was conducted using both conventional stains and dynamic D-FF-OCT imaging. Results: D-FF-OCT enabled the recognition of most kidney structures. The results showed a significant correlation between this technology and conventional stains for the evaluation of both interstitial fibrosis (IF) (r = 0.61, P < 0.001) and tubular atrophy (TA) (r = 0.60, P < 0.001). Although many lesions could be identified such as interstitial inflammation, acute tubular necrosis, glomerular crescents, and vascular intimal thickening; other recognitions such as glomerular membranous deposits, vascular amyloidosis, and peritubular capillaritis will require confirmation in larger cohorts. Conclusion: This study demonstrates the potential of D-FF-OCT imaging for on-site analysis of kidney biopsies, providing rapid and high-resolution images without extensive sample preparation.
Multi-spectral reflection matrix for ultrafast 3D label-free microscopy Balondrade, P., V. Barolle, N. Guigui, E. Auriant, N. Rougier, C. Boccara, M. Fink, and A. Aubry Nature Photonics 18, no. 10, 1097-1104 (2024) Résumé: Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit the penetration depth. Multi-conjugate adaptive optics and time-gated matrix approaches have been developed to compensate for aberrations but the associated frame rate is extremely limited for three-dimensional imaging. Here we develop a multi-spectral matrix approach to solve these fundamental problems. On the basis of a sparse illumination scheme and an interferometric measurement of the reflected wave field at multiple wavelengths, the focusing process can be optimized in post-processing for any voxel by addressing independently each frequency component of the reflection matrix. A proof-of-concept experiment shows a three-dimensional image of an opaque human cornea over a 0.1 mm3 field of view at a 290 nm resolution and a 1 Hz frame rate. This work paves the way towards a fully digital microscope allowing real-time, in vivo, quantitative and deep inspection of tissues.

Optophysiology using dynamic full-field optical coherence tomography (D-FF-OCT) Norberg, N., S. Azzollini, T. Monfort, J. Granier, O. Thouvenin, and K. Grieve Progress in Biomedical Optics and Imaging - Proceedings of SPIE 13305, 16 (2025) Résumé: We present an approach for assessing photoreceptor function using dynamic full-field optical coherence tomography (DFF-OCT) in an ex-vivo macaque retinal explant. Our non-invasive method reveals photoreceptor function through high-resolution (0.4 µm transverse, 0.7 µm axial) D-FF-OCT imaging, coupled with a signal processing pipeline that quantifies changes in photoreceptor intensity variability relative to pre-photostimulation levels. Following dark adaptation, photoreceptor responses to a single green light flash (530 nm, 100 ms) were recorded and analyzed. Regions of interest (ROIs) were segmented using Cellpose and classified as responders or non-responders based on intensity thresholds relative to pre-stimulation variability. Of the photoreceptor cells analyzed (n=1075), 62% were identified as responders, showing a significant increase in intensity variability compared to controls. The optophysiology curve revealed a rapid divergence in stimulated and control conditions post-flash, underscoring the ability of D-FF-OCT to capture light-induced functional changes. This study demonstrates that optophysiology using D-FF-OCT offers a robust, non-invasive alternative for photoreceptor functional assessment.
Rolling-phase dynamic full-field OCT Monfort, T., K. Grieve, and O. Thouvenin Optics Letters 50, no. 7, 2239-2242 (2025) Résumé: Dynamic full-field optical coherence tomography (DFFOCT) has recently emerged as an invaluable label-free microscopy technique, owing to its sensitivity to cell activity, as well as speed and sectioning ability. However, the quality of DFFOCT images is often degraded due to phase noise and fringe artifacts. In this work, we present a new implementation, to the best of our knowledge, named rolling-phase (RP) DFFOCT, in which the reference arm is slowly scanned over magnitudes exceeding 2π. We demonstrate mathematically and experimentally that it shows superior image quality while enabling to extract both static and dynamic contrast simultaneously. We showcase RP-DFFOCT on a macaque retinal explant and demonstrate its ability to better resolve subcellular structures, including intranuclear activity.

3D Single-Molecule Super-Resolution Imaging of Microfabricated Multiscale Fractal Substrates for Calibration and Cell Imaging Cabriel, C., R. M. Córdova-Castro, E. Berenschot, A. Dávila-Lezama, K. Pondman, S. Le Gac, N. Tas, A. Susarrey-Arce, and I. Izeddin ACS Applied Materials and Interfaces 17, no. 6, 9019-9034 (2025) Résumé: Microstructures arrayed over a substrate have shown increasing interest due to their ability to provide advanced 3D cellular models, which open up new possibilities for cell culture, proliferation, and differentiation. Still, the mechanisms by which physical cues impact the cell phenotype are not fully understood, hence the necessity to interrogate cell behavior at the highest resolution. However, cell 3D high-resolution optical imaging on such microstructured substrates remains challenging due to their complexity as well as axial calibration issues. In this work, we address this issue by leveraging the geometrical characteristics of fractal-like structures, which serve as axial calibration tools and modulate cell growth. To this end, we use multiscale 3D SiO2 substrates consisting of spatially arrayed octahedral features of a few micrometers to hundreds of nanometers. Through optimizations of both the structures and optical imaging conditions, we demonstrate the potential of these 3D multiscale structures as an alternative to electron microscopy for material imaging but also as calibration tools for 3D super-resolution microscopy. We used their multiscale and known geometry to perform lateral and axial calibrations in 3D single-molecule localization microscopy (SMLM) and assess imaging resolutions. We then utilized these substrates as a platform for high-resolution bioimaging. As a proof of concept, we cultivate human mesenchymal stem cells on these substrates, revealing very different growth patterns compared to flat glass. Specifically, the spatial distribution of cytoskeleton proteins is vastly modified, as we demonstrate with a 3D SMLM assessment. Mots-clés: 3D single-molecule localization microscopy; bioimaging; multiscale material; fractal-like microstructures; calibration; material imaging
Isotope effects and temperature-dependence studies on vibrational lifetimes of interstitial oxygen in silicon Kohli, K. K., G. Davies, N. Q. Vinh, D. West, S. K. Estreicher, T. Gregorkiewicz, I. Izeddin, and K. M. Itoh Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 253, no. 1-2, 200-204 (2006)

Group Members

Permanent Researchers
– Sébastien BIDAULT
– Claude BOCCARA
– Emmanuel FORT
– Ignacio IZEDDIN

Post-doctoral Researchers
– Daria ANDREOLI
– Egidijus AUKSORIUS
– Charles-Edouard LEROUX
– Nemanja MARKESEVIC
– Thu-Mai NGUYEN

PhD Students
– Clement APELIAN
– Vincent BACOT
– Olivier THOUVENIN
– Peng XIAO

The Langevin Institute

News

Research

People

Publications

Seminars & workshops

Teaching

Now hiring

Contact

Biophotonics

Recent publications

Group Members

Rubriques