Ignacio IZEDDIN

Ignacio IZEDDIN

Associate professor at ESPCI
Tel.: +33 (0)1 80 96 30 75
Room: R66
Mail: ignacio.izeddin (arobase) espci.fr


Keywords
Single-molecule localization microscopy (SMLM); super-resolution imaging; single-particle tracking (SPT); biophotonics; diffusion processes in cell biology; molecular cell biology; transcription regulation; DNA repair; light-matter interactions at the nanoscale

Research activity

Welcome to my research page.

I am Ignacio Izeddin, an Associate Professor at Institut Langevin, ESPCI Paris, CNRS, PSL University. As a physicist, my research lies at the intersection of advanced optical imaging, biophysics and cell biology. Driven by a fundamental curiosity about the physical principles that govern biological functions, I focus on developing microscopy tools to illuminate the intricate spatial organization and dynamic behavior of living cells.

My research centers on advancing single-molecule localization microscopy (SMLM) to push the boundaries of what we can visualize and comprehend about the molecular distribution and dynamics of cells. I am particularly keen on using super-resolution imaging and single-particle tracking to explore how cells regulate complex processes such as transcription and DNA repair at the molecular level.

By developing innovative microscopy tools, my work aims to overcome current limitations in spatial and temporal resolution. These advancements help us capture fast, dynamic processes, offering a clearer picture of how proteins interact and function within the cell’s intricate environment. Ultimately, my goal is to provide deeper insights into cellular function that can contribute to fields such as gene regulation, DNA repair mechanisms, and other areas of molecular biology through the application of these advanced imaging techniques.

Open positions in the lab (updated February 2025)

PhD Offer: Synuclein diffusion analysis in neurons using event-based super-resolution microscopy (SEVEN)

  • This project is co-directed by Christian SPECHT (NeuroBicêtre / DHNS, University Paris-Saclay) and our team at the Institut Langevin, ESPCI Paris-PSL, CNRS. The main location of the PhD candidate will be at NeuroBicêtre / DHNS
  • This is a LIGHTinParis COFUND European project, with certain criteria for eligibility including international mobility
  • More details in the website of the project

We are seeking a motivated PhD student with a background in neuroscience and/or physics, and a strong interest in the molecular mechanisms of cell biological phenomena. The project is co-directed by Christian SPECHT (NeuroBicêtre / DHNS, University Paris-Saclay) and Ignacio IZEDDIN and Clément CABRIEL (Institut Langevin, PSL University). The PIs have substantial experience in teaching and supervision at PhD and postdoctoral level. The project brings together two interdisciplinary groups with complementary expertise in the fields of Neuroscience and Physics, in line with the 3i requirement of the COFUND call. Christian Specht is a neuroscientist with a longstanding interest in the molecular interactions and diffusion processes at synapses. Ignacio Izeddin is a physicist specialising in advanced imaging technologies. Together with Clément CABRIEL (Institut Langevin), he has pioneered the use of event-based sensors for single molecule imaging which is essential to this project.

Software/data processing engineer on Event-based SMLM

We invite applications for a software or data processing engineer position to work in an interdisciplinary team on an innovative technology integrating event-based sensors (EBS) into single-molecule localization microscopy (SMLM) [1]. Our research aims to develop new methods to enable time-resolved high-resolution imaging of proteins in living biological samples. EBS technology offers an asynchronous response to changes in light intensity, making it uniquely suited to capture fast, multiscale biological processes.

Following our proof of principle of this approach, we aim to develop tools to simplify and standardize acquisitions and data processing. In particular, we would like to develop an acquisition software capable of controlling the acquisition instruments and displaying a rendering of the results in real time. We also plan to improve the post-acquisition data processing pipeline to make it more user-friendly, improve processing speed and develop visualization tools.

[1] C. Cabriel, T. Monfort, C.G. Specht, and I. Izeddin. Event-based vision sensor for fast and dense single-molecule localization microscopy. Nat. Photon. 17, 1105–1113 (2023). https://doi.org/10.1038/s41566-023-01308-8

Publications

3D Single-Molecule Super-Resolution Imaging of Microfabricated Multiscale Fractal Substrates for Calibration and Cell Imaging
Cabriel, C., R. M. Córdova-Castro, E. Berenschot, A. Dávila-Lezama, K. Pondman, S. Le Gac, N. Tas, A. Susarrey-Arce, and I. Izeddin
ACS Applied Materials and Interfaces 17, no. 6, 9019-9034 (2025)
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Single-emitter super-resolved imaging of radiative decay rate enhancement in dielectric gap nanoantennas
Córdova-Castro, R. M., B. Van Dam, A. Lauri, S. A. Maier, R. Sapienza, Y. De Wilde, I. Izeddin, and V. Krachmalnicoff
Light: Science and Applications 13, no. 1 (2024)
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3D topographies promote macrophage M2d-Subset differentiation
Carrara, S. C., A. Davila-Lezama, C. Cabriel, E. J. W. Berenschot, S. Krol, J. G. E. Gardeniers, I. Izeddin, H. Kolmar, and A. Susarrey-Arce
Materials Today Bio 24 (2024)
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3D-Architected Alkaline-Earth Perovskites
Winczewski, J. P., J. Arriaga Dávila, M. Herrera-Zaldívar, F. Ruiz-Zepeda, R. M. Córdova-Castr, C. R. Pérez De La Vega, C. Cabriel, I. Izeddin, H. Gardeniers, and A. Susarrey-Arce
Advanced Materials (2024)
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Event-based vision sensor for fast and dense single-molecule localization microscopy
Cabriel, C., T. Monfort, C. G. Specht, and I. Izeddin
Nature Photonics 17, no. 12, 1105-1113 (2023)
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Real-time detection of virus antibody interaction by label-free common-path interferometry
Alhaddad, S., H. Bey, O. Thouvenin, P. Boulanger, C. Boccara, M. Boccara, and I. Izeddin
Biophysical Reports 3, no. 3, 100119 (2023)
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Des nanotorches pour étudier les matériaux nanostructurés
Izeddin, I., and V. Krachmalnicoff
Photoniques, no. 114, 40-44 (2022)
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Additive Manufacturing of 3D Luminescent ZrO2:Eu3+ Architectures
Winczewski, J., M. Herrera, C. Cabriel, I. Izeddin, S. Gabel, B. Merle, A. Susarrey Arce, and H. Gardeniers
Advanced Optical Materials, 2102758 (2022)
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Super-resolution imaging: When biophysics meets nanophotonics
Koenderink, A. F., R. Tsukanov, J. Enderlein, I. Izeddin, and V. Krachmalnicoff
Nanophotonics 11, no. 2, 169-202 (2022)
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Epigenetic rewriting at centromeric DNA repeats leads to increased chromatin accessibility and chromosomal instability
Decombe, S., F. Loll, L. Caccianini, K. Affannoukoué, I. Izeddin, J. Mozziconacci, C. Escudé, and J. Lopes
Epigenetics and Chromatin 14, no. 1 (2021)
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Anomalous Subdiffusion in Living Cells: Bridging the Gap Between Experiments and Realistic Models Through Collaborative Challenges
Woringer, M., I. Izeddin, C. Favard, and H. Berry
Frontiers in Physics 8 (2020)
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Relocating Single Molecules in Super-Resolved Fluorescence Lifetime Images near a Plasmonic Nanostructure
Blanquer, G., B. Van Dam, A. Gulinatti, G. Acconcia, Y. De Wilde, I. Izeddin, and V. Krachmalnicoff
ACS Photonics 7, no. 2, 393-400 (2020)
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Cramér-Rao analysis of lifetime estimations in time-resolved fluorescence microscopy
Bouchet, D., V. Krachmalnicoff, and I. Izeddin
Optics Express 27, no. 15, 21239-21252 (2019)
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Probing near-field light-matter interactions with single-molecule lifetime imaging
Bouchet, D., J. Scholler, G. Blanquer, Y. De Wilde, I. Izeddin, and V. Krachmalnicoff
Optica 6, no. 2, 135-138 (2019)
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One-Shot Measurement of the Three-Dimensional Electromagnetic Field Scattered by a Subwavelength Aperture Tip Coupled to the Environment
Rahbany, N., I. Izeddin, V. Krachmalnicoff, R. Carminati, G. Tessier, and Y. De Wilde
ACS Photonics 5, no. 4, 1539-1545 (2018)
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Multi-scale tracking reveals scale-dependent chromatin dynamics after DNA damage
Miné-Hattab, J., V. Recamier, I. Izeddin, R. Rothstein, and X. Darzacq
Molecular Biology of the Cell 28, no. 23, 3323-3332 (2017)
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Single molecule study of non-specific binding kinetics of LacI in mammalian cells
Caccianini, L., D. Normanno, I. Izeddin, and M. Dahan
Faraday Discussions 184, 393-400 (2015)
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Accessing the third dimension in localization-based super-resolution microscopy
Hajj, B., M. El Beheiry, I. Izeddin, X. Darzacq, and M. Dahan
Phys. Chem. Chem. Phys. 16, no. 31, 16340-16348 (2014)
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Geometry of the nucleus: a perspective on gene expression regulation
Woringer, M., X. Darzacq, and I. Izeddin
Current Opinion in Chemical Biology 20, 112-119 (2014)
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Single-molecule tracking in live cells reveals distinct target-search strategies of transcription factors in the nucleus
Izeddin, I., V. Récamier, L. Bosanac, I. I. Cissé, L. Boudarene, C. Dugast-Darzacq, F. Proux, O. Bénichou, R. Voituriez, O. Bensaude, M. Dahan, and X. Darzacq
eLife 3 (2014)
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The SNARE Sec22b has a non-fusogenic function in plasma membrane expansion
Petkovic, M., A. Jemaiel, F. Daste, C. G. Specht, I. Izeddin, D. Vorkel, J.-M. Verbavatz, X. Darzacq, A. Triller, K. H. Pfenninger, D. Tareste, C. L. Jackson, and T. Galli
Nature Cell Biology 16, no. 5, 434-444 (2014)
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Single cell correlation fractal dimension of chromatin
Récamier, V., I. Izeddin, L. Bosanac, M. Dahan, F. Proux, and X. Darzacq
Nucleus 5, no. 1, 75-84 (2014)
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Real-Time Dynamics of RNA Polymerase II Clustering in Live Human Cells
Cisse, I. I., I. Izeddin, S. Z. Causse, L. Boudarene, A. Senecal, L. Muresan, C. Dugast-Darzacq, B. Hajj, M. Dahan, and X. Darzacq
Science 341, no. 6146, 664-667 (2013)
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Quantitative Nanoscopy of Inhibitory Synapses: Counting Gephyrin Molecules and Receptor Binding Sites
Specht, C., I. Izeddin, P. Rodriguez, M. El beheiry, P. Rostaing, X. Darzacq, M. Dahan, and A. Triller
Neuron 79, no. 2, 308-321 (2013)
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PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking
Izeddin, I., M. El Beheiry, J. Andilla, D. Ciepielewski, X. Darzacq, and M. Dahan
Optics Express 20, no. 5, 4957 (2012)
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Wavelet analysis for single molecule localization microscopy
Izeddin, I., J. Boulanger, V. Racine, C. G. Specht, A. Kechkar, D. Nair, A. Triller, D. Choquet, M. Dahan, and J. B. Sibarita
Optics Express 20, no. 3, 2081 (2012)
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Assessing the localization of centrosomal proteins by PALM/STORM nanoscopy
Sillibourne, J. E., C. G. Specht, I. Izeddin, I. Hurbain, P. Tran, A. Triller, X. Darzacq, M. Dahan, and M. Bornens
Cytoskeleton 68, no. 11, 619-627 (2011)
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Lateral Diffusion on Tubular Membranes: Quantification of Measurements Bias
Renner, M., Y. Domanov, F. Sandrin, I. Izeddin, P. Bassereau, and A. Triller
PLoS ONE 6, no. 9, e25731 (2011)
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Microscopies cellulaires à l’échelle de la molécule individuelle
Izeddin, I., X. Darzacq, and M. Dahan
médecine/sciences 27, no. 5, 547-552 (2011)
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Super-Resolution Dynamic Imaging of Dendritic Spines Using a Low-Affinity Photoconvertible Actin Probe
Izeddin, I., C. G. Specht, M. Lelek, X. Darzacq, A. Triller, C. Zimmer, and M. Dahan
PLoS ONE 6, no. 1, e15611 (2011)
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Saturation of luminescence from Si nanocrystals embedded in SiO<sub>2</sub>
Timmerman, D., I. Izeddin, and T. Gregorkiewicz
physica status solidi (a) 207, no. 1, 183-187 (2010)
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Energy transfer in Er-doped SiO2 sensitized with Si nanocrystals
Izeddin, I., D. Timmerman, T. Gregorkiewicz, A. S. Moskalenko, A. A. Prokofiev, I. N. Yassievich, and M. Fujii
Physical Review B 78, no. 3 (2008)
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Space-separated quantum cutting with silicon nanocrystals for photovoltaic applications
Timmerman, D., I. Izeddin, P. Stallinga, I. N. Yassievich, and T. Gregorkiewicz
Nature Photonics 2, no. 2, 105-109 (2008)
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Mid-infrared spectroscopy of the Er-related donor state in Si/Si:Er3+ nanolayers
Izeddin, I., M. A. J. Klik, N. Q. Vinh, M. S. Bresler, and T. Gregorkiewicz
Materials Science and Engineering: B 146, no. 1-3, 131-134 (2008)
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Donor-State-Enabling Er-Related Luminescence in Silicon: Direct Identification and Resonant Excitation
Izeddin, I., M. A. J. Klik, N. Q. Vinh, M. S. Bresler, and T. Gregorkiewicz
Physical Review Letters 99, no. 7 (2007)
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Er-Doped Electro-Optical Memory Element for 1.5 um Silicon Photonics
Gregorkiewicz, T., B. A. Andreev, M. Forcales, I. Izeddin, W. Jantsch, Krasil', Z. F. nik, D. I. Kryzhkov, V. P. Kuznetsov, and J. M. Zavada
IEEE Journal of Selected Topics in Quantum Electronics 12, no. 6, 1539-1544 (2006)
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Non-radiative sub-microsecond recombination of excited Er3+ ions in SiO2 sensitized with Si nanocrystals
Izeddin, I., T. Gregorkiewicz, and M. Fujii
Physica E: Low-dimensional Systems and Nanostructures 38, no. 1-2, 144-147 (2007)
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Nanosecond dynamics of the near-infrared photoluminescence of Er-doped SiO2 sensitized with Si nanocrystals
Izeddin, I., A. S. Moskalenko, I. N. Yassievich, M. Fujii, and T. Gregorkiewicz
Physical Review Letters 97, no. 20 (2006)
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Isotope effects and temperature-dependence studies on vibrational lifetimes of interstitial oxygen in silicon
Kohli, K. K., G. Davies, N. Q. Vinh, D. West, S. K. Estreicher, T. Gregorkiewicz, I. Izeddin, and K. M. Itoh
Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 253, no. 1-2, 200-204 (2006)
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Isotope dependence of the lifetime of the 1136 cm-1 vibration of oxygen in silicon
Kohli, K. K., G. Davies, N. Q. Vinh, D. West, S. K. Estreicher, T. Gregorkiewicz, I. Izeddin, and K. M. Itoh
Physical Review Letters 96, no. 22 (2006)
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On 2.7 um Emission from Er-doped Large Bandgap Hosts
Vrielinck, H., I. Izeddin, V. Y. Ivanov, T. Gregorkiewicz, F. Callens, D. S. Lee, A. J. Steckl, and N. M. Khaidukov
MRS Proceedings 866 (2005)
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Photoluminescence and excitation spectroscopy of the 1.5  μm Er-related band in MBE-grown GaN layers
Izeddin, I., T. Gregorkiewicz, D. S. Lee, and A. J. Steckl
Superlattices and Microstructures 36, no. 4-6, 701-705 (2004)
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Excitation paths in RE-doped III–V semiconductors
Klik, M. A. J., I. Izeddin, J. Phillips, and T. Gregorkiewicz
Materials Science and Engineering: B 105, no. 1-3, 141-145 (2003)
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