Ultrafast retinal Doppler holography (https://arxiv.org/abs/1804.10066) is an innovative noninvasive functional exam of the retinal microcirculation. Performed in a few seconds on an undilated eye with diffuse near-infrared illumination (https://arxiv.org/abs/2212.13347), this exam reconstructs arterial and venous flow-velocity waves that reflect variations in ocular perfusion pressure (https://arxiv.org/abs/2409.17180). This approach provides access to dynamical metrics (blood-flow volume, vessel-wall motion), mechanical metrics (resistance, compliance, elasticity), and rheological metrics (viscosity, wall shear stress) unprecedented in clinical practice. An open-source software pipeline enables high-throughput, real-time acquisition (https://arxiv.org/abs/2508.03911, https://github.com/DigitalHolography/Holovibes), image rendering with aberration compensation (https://doi.org/10.1364/BOE.528568), automated extraction of quantitative metrics and biomarkers (https://github.com/DigitalHolography/HoloDoppler), and generation of a standardized clinical report (https://github.com/DigitalHolography/EyeFlow/)—the foundation for ongoing multicenter clinical studies across the United States, Europe, and China.
These rigorous, standardized metrics offer powerful tools for triage, personalized treatment adjustment, and longitudinal follow-up of patients and large-scale cohorts. They directly inform studies of major blinding diseases such as glaucoma, diabetic retinopathy, retinal vein occlusion, and ocular ischemia, while extending their relevance to systemic conditions such as cardiovascular disease. Tracking the hemodynamic, mechanical, and rheological signatures of the retinal vascular bed provides an objective language of microvascular health and vascular risk. Our ambition is to identify and validate biomarkers that enable intervention at the earliest stages of microvascular impairment, when physiology remains reversible, to prevent the onset of structural lesions rather than observe them a posteriori.
As part of this project, the internship will focus on quantitative image analysis of retinal Doppler holography data using Python and MATLAB. The intern will contribute to the development and validation of automated data-driven processing pipelines for extracting flow-related biomarkers, including blood-flow velocity profiles, vessel-wall dynamics, and rheological metrics. This work will involve implementing signal and image processing algorithms, performing statistical analyses on large datasets, and validating results against clinical use cases. The internship will also provide the opportunity to collaborate with physicists, engineers, and clinicians, gaining experience at the interface of computational imaging and medical research.
contact :
Michael Atlan
https://www.linkedin.com/in/micatlan
Source code repository : github.com/DigitalHolography
References
Diffuse laser illumination for Maxwellian view Doppler holography of the retina
https://arxiv.org/abs/2212.13347
Full-field swept-source optical coherence tomography and laser Doppler holography :
http://arxiv.org/abs/2112.08494
Anterior segment, blood flow imaging, eye tracking, and transparency assessment :
https://arxiv.org/abs/2107.10799
Blood flow reversal in out-of-plane vessels :
https://arxiv.org/abs/2008.09813
Reverse contrast laser Doppler holography :
https://arxiv.org/abs/2004.00007
Real-time principal component analysis :
https://arxiv.org/abs/2004.00923
Spatio-temporal filtering :
https://arxiv.org/abs/2003.10259
Waveform analysis of human retinal and choroidal blood flow with laser Doppler holography :
https://arxiv.org/abs/2106.00634
Choroidal vasculature imaging with laser Doppler holography :
https://arxiv.org/abs/2106.00608
Swept-source optical coherence tomography by digital holography in real-time :
https://arxiv.org/abs/2003.08960
Doppler holography of the human retina :
https://arxiv.org/abs/1804.10066
High speed optical holography of retinal blood flow :
https://arxiv.org/abs/1607.07800
Doppler imaging of microvascular blood flow :
https://arxiv.org/abs/1412.0580
Holographic laser Doppler ophthalmoscopy :
https://arxiv.org/abs/1006.2604
