Résumé: High refractive index dielectric nanoantennas strongly modify the decay rate via the Purcell effect through the design of radiative channels. Due to their dielectric nature, the field is mainly confined inside the nanostructure and in the gap, which is hard to probe with scanning probe techniques. Here we use single-molecule fluorescence lifetime imaging microscopy (smFLIM) to map the decay rate enhancement in dielectric GaP nanoantenna dimers with a median localization precision of 14 nm. We measure, in the gap of the nanoantenna, decay rates that are almost 30 times larger than on a glass substrate. By comparing experimental results with numerical simulations we show that this large enhancement is essentially radiative, contrary to the case of plasmonic nanoantennas, and therefore has great potential for applications such as quantum optics and biosensing.

Résumé: Shear stress plays a critical role in regulating physiological processes within microcirculatory systems. While particle imaging velocimetry is a standard technique for quantifying shear flow, uncertainty near boundaries and low resolution remain severe restrictions. Additionally, shear stress determination is particularly challenging in biofluids due to their significant non-Newtonian behaviors. The present study develops a shearmetry technique in physiological settings using a biomimetic fluid containing rare earth-doped luminescent nanorods acting in two roles. First, they are used as colloidal additives adjusting rheological properties in physiological media. Their anisotropic morphology and interparticle interaction synergistically induce a non-Newtonian shear-thinning effect emulating real biofluids. Second, they can probe shear stress due to the shear-induced alignment. The polarized luminescence of the nanorods allows for quantifying their orientational order parameter and thus correlated shear stress. Using scanning confocal microscopy, we demonstrate the tomographic mapping of the shear stress distribution in microfluidics. High shear stress is evident near the constriction and the cellular periphery, in which non-Newtonian effects can have a significant impact. This emerging shearmetry technique is promising for implementation in physiological and rheological environments of biofluids.

Résumé: Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit the penetration depth. Multi-conjugate adaptive optics and time-gated matrix approaches have been developed to compensate for aberrations but the associated frame rate is extremely limited for three-dimensional imaging. Here we develop a multi-spectral matrix approach to solve these fundamental problems. On the basis of a sparse illumination scheme and an interferometric measurement of the reflected wave field at multiple wavelengths, the focusing process can be optimized in post-processing for any voxel by addressing independently each frequency component of the reflection matrix. A proof-of-concept experiment shows a three-dimensional image of an opaque human cornea over a 0.1 mm3 field of view at a 290 nm resolution and a 1 Hz frame rate. This work paves the way towards a fully digital microscope allowing real-time, in vivo, quantitative and deep inspection of tissues.